"Now, it's the time that they (US) should do more (for the war on terrorism)," Gilani told reporters on the sidelines of a function at a girls' college in Islamabad.
During the past decade, Pakistan had "sacrificed much in battling the menace of terrorism" and the country should not be "pressurized to do more", he said.
The premier's remarks came a day after Pakistan's Foreign Office rejected a warning by US Defense Secretary Leon Panetta that Washington would retaliate against attacks by Pakistan-based militants.
The Foreign Office spokesperson Tehmina Janjua said Panettas remarks were "out of line" with ongoing counter- terrorism cooperation between the two sides.